Histoarchitecture and Histochemical Studies on the Lacrimal Gland of Pig (Sus scrofa)

The fresh tissues were collected from five pigs of either sex and processed for histomorphological studies. The gland was located on dorso-lateral aspect of the eye in the orbital cavity. The gland was multilobar tubulo-acinar with predominance of acini. It was covered by a thick connective tissue capsule and connective tissue septa, which penetrate into the glandular parenchyma and divided the gland into lobes and lobules. The acini were of mixed type but predominantly sero-mucous in nature. The secretory units were followed by a duct system. The excretory duct system was made up of intercalated, intralobular, interlobular and large excretory ducts. The strong presence of glycogen in the acini was demonstrated with the help PAS (McManus’) stain. The ducts showed very weak reaction for the presence of glycogen but goblet cells in the stratified ducts showed strong positive reaction for PAS. The acini showed strong reaction for acidic mucosubstances with PAS-AB. The goblet cells in the stratified ducts also showed strong positive activity towards the PAS-AB. The intense activity towards the Alcian blue showed presence of sialomucins and hyaluronic acids in glandular parenchyma of the lacrimal gland

The Ultra Structural Studies of Duodenum of Goat (Capra hircus)

The tissues from small intestine containing duodenum were collected from six young goats and processed for scanning and transmission electron microscopic studies. The villi of the duodenum varied in shape and size. The duodenal mucosal surface showed leaf shaped villi along with scanty population of goblet cells on the villi surface. The mucosal surface was having very faint corrugations and dense mat of microvilli. The basal surface of the villi, the number of goblet cells was more. The crypts openings were evident at some places. The transmission electron microscopic studies revealed the presence of different cell population which included columnar or absorptive cells and goblet cells in surface epithelium of the duodenum. The columnar cells were having many uniform regularly spaced microvilli. The goblet cells were very few in the villus epithelium and were interspersed in between the columnar cells and their cytoplasm was distended with mucus granules. The crypt region or glandular epithelium was consisted of polymorphic cell population which included the Paneth cells, enterochromaffin cells and goblet cells